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However, the current GSA11 tools utilize over representation analysis,12 which reports the enrichment of functional groups (for example, gene sets) for the genes of interest. They compromise the connectivity in favor of computational simplicity that is based on cellular components and not their connectivity.3,4 In other words, current tools do not analyze the wiring diagram of the interactions (for example, activation, inhibition) in a functional molecular network.13We have developed PATHOME (pathway and transcriptome information), a novel computational algorithm for identifying differentially expressed subpathways.
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